iPSC-Derived MSCs that Are Genetically Engineered for Systemic Bone Augmentation

Abstract

Mesenchymal stem cells (MSCs) differentiated from Induced pluripotent stem cells (iPSCs) have a potential application in clinic to treat osteoporosis and other skeletal diseases. Further engineering MSCs with homing factors like CXCR4 and osteogenic factors like shNoggin or FGF2 may increase the therapeutic effects. Toward these goals, we have generated iPSCs using lentiviral vectors from blood cells and integration-free iPSCs using episomal vectors. The generated iPSCs are pluripotent, as evident by expression of pluripotency markers and formation of teratoma. After differentiation of iPSCs into MSCs, the cells express MSC markers and can form bone nodule in in vitro culture. We will further test in year 2 whether iPSC-Derived MSCs that are genetically engineered with CXCR4 and other factors can increase systemic bone formation after transplantation in mice. We also proposed an alternative strategy for generating MSCs: direct conversion of blood cells into MSCs rather than reprogramming blood cells into iPSCs followed by re-differentiation.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2012
Accession Number
ADA568164

Entities

People

  • Xiaobing Zhang

Organizations

  • Loma Linda University

Tags

DTIC Thesaurus Topics

  • Antigens
  • Blood
  • Blood Cells
  • Bone Marrow Cells
  • Cells
  • Confocal Microscopy
  • Culture Techniques
  • Embryos
  • Hematopoietic Cells
  • Medical Personnel
  • Regenerative Medicine
  • Stem Cells
  • Tissues

Fields of Study

  • Biology
  • Chemistry

Readers

  • Immunology and Pathology
  • Molecular and Cellular Biology
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology