The Role of Crk Adaptor Proteins in Breast Tumorigenesis and Bone Metastasis

Abstract

We have established a relationship between Crk protein expression and cell proliferation in basal breast cancer. Stable knockdown of Crk protein expression using shRNA in multiple basal breast cancer cell lines decreased both adhesion-independent growth in vitro and proliferation in vivo. Immunohistochemistry to CrkI/II, CrkL, and the proliferative marker Ki67 revealed a positive correlation between Crk protein levels and the proliferative index of human basal tumors in a tissue microarray. The association of a Crk gene expression signature with basal tumors and poor prognosis further supports a role for Crk-dependent signaling in the growth of basal tumors. These findings support a requirement for Crk protein expression in basal tumor progression, and identify Crk proteins as an attractive subject for further study and therapeutic intervention in basal tumors.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2012
Accession Number
ADA568434

Entities

People

  • Emily Bell

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cancer
  • Carcinoma
  • Cell Line
  • Cell Movement
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Confocal Microscopy
  • Gene Expression
  • Health Services
  • Medical Personnel
  • Neoplasms
  • Oncology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Oncology and Biomarker-Based Cancer Detection.