Global Characterization of Protein-Altering Mutations in Prostate Cancer
Abstract
The identification of recurrent, protein-altering genetic alterations is frequently the means by which a given gene is initially implicated in tumor biology. Our specific aims are: (1) Carry out the genome-wide identification of nonsynonymous mutations in prostate metastases; (2) Evaluate the mutational histories of individual mutations within the progression of the cancer in which it was observed, and assess prevalence; (3) Perform integrative analyses of somatic mutation with gene expression and copy number change data collected on the same samples. During this funding period we have: (i) completed the identification and quality assessment of the prostate cancer tissues/samples that will be used for the project; (ii) completed DNA isolation for 300 samples/cases of prostate cancer; (iii) Transfered the 1 st and 2 nd groups of DNAs to the Shendure lab for Exome analysis; (iv) completed exome sequencing on 50 tumor metastasis and completed the analysis of 27 of these exomes; (v) completed CGH assays on 200 prostate cancers; (vi) initiated the integrated analyses of gene expression, copy number and mutation in prostate cancer using network approaches.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2012
- Accession Number
- ADA568599
Entities
People
- Peter S Nelson
Organizations
- Fred Hutchinson Cancer Center