Selective Gene Regulation by Androgen Receptor in Prostate Cancer
Abstract
Androgen binds to the androgen receptor (AR) and is required for prostate cancer initiation and progression. Although androgen ablation is initially an effective prostate cancer therapy, patients eventually develop resistance, but disease remains driven by AR. There is a need to identify AR gene programs that promote proliferation versus differentiation to design better treatments. Using mutant ARs as models of aberrant AR activity, I generated cell lines harboring wild-type and mutant ARs and analyzed proliferation, anchorage-independent cell growth and alterations in AR target genes. I also generated a multiplexed promoter assay for high-throughput screening (HTS) to identify compounds that selectively regulate AR. After assay optimization, the primary HTS was performed with 2,5000 compounds. A dose response assay identified several compounds that selectively regulate AR and will be further studied in cell-based assays.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2012
- Accession Number
- ADA568972
Entities
People
- Elizabeth Lapensee
Organizations
- University of Michigan