Rapid Dispersion of Polymicrobial Wound Biofilms with Depolymerase Enzymes
Abstract
During the performance period, we successfully developed protocols to extract biofilm EPS associated with ESKAPE organisms (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa, and Enterobacter species). We further performed composition and linkage analysis on these samples and determined that 2 and 3-linked mannose residues constitute the major carbohydrate moiety in the EPS. In parallel, we synthesized, expressed, purified, and characterized 15 putative depolymerase enzymes by a variety of assays against static, dynamic, and mixed biofilm conditions. At least four enzymes (DSPB, NagZ, EQ, and Betty) will be advanced to safety and toxicity testing in the next performance period.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2012
- Accession Number
- ADA569015
Entities
People
- Daniel C Nelson
Organizations
- University of Maryland