Regulation of c-Myc mRNA by L11 in Response to UV and Gamma Irradiation
Abstract
In previous funding year, we have discovered a novel regulatory paradigm wherein L11 plays a critical role in controlling c-myc mRNA turnover via recruiting miR-24-loaded miRISC to the c-myc mRNA 3 -UTR in response to ribosomal stress. We show that ribosome-free L11 binds to c-myc mRNA in the cytoplasm and this binding is enhanced in response to ribosomal stress. Meanwhile, we found that c-myc mRNA is also down-regulated in response to DNA damage including UV and -irradiation in an L11-dependent manner. In current funding year, we have explored the role of miR-130a in DNA damage-mediated c-myc down-regulation. We provided evidence indicating that miR-130a directly targets c-myc mRNA. UV treatment enhances the association of L11, Ago2, as well as miR-130a with the c-myc mRNA. Together, our current results suggest that L11 may recruit miR-130a-loaded miRISC to mediate c-myc decay in response to UV-induced DNA damage and implying that miR-130a may possesses a growthinhibitory function through down regulating c-Myc.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2012
- Accession Number
- ADA569164
Entities
People
- Mu-Shui Dai
Organizations
- Oregon Health & Science University