The Role of Tumor Associated Macrophage in Recurrent Growth of Tumor Stem Cell
Abstract
According to the recent cancer stem cell (CSC) theory, recurrent tumor must arise from a dormant tumor stem cell whose re- growth is triggered by shifting of microenvironment. This project aims at clarifying the roles of TAM in recurrent growth of dormant stem cell in breast cancer. We hypothesize that the balance of dormancy and recurrence is determined by the ability of the tumor stem cells to recruit TAM which in turn promotes self-renewal of the stem cell. We have established necessary mouse colonies and also developed the method to generate TAM. We have also shown that TAM indeed promoted the growth of CSCs in our animal model. Due to the relocation of our entire lab, Task 2 which involves extensive animal breeding has been delayed; however, we have compensated the lack of progress in Task 2 by further extending our mechanistic study in Task 1. We have shown that the interaction of TAM with CSCs lead to enhanced secretion of PDGF-BB from TAMs which then activated stromal cells and enhanced CSC self-renewal. We believe that the outcome of our results set a new paradigm in our understanding of metastatic niche of CSCs in the bone, which may provide novel targets such as TAM, PDGF and FGF, for the treatment of recurrent disease.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2012
- Accession Number
- ADA569375
Entities
People
- Kounosuke Watabe
Organizations
- Southern Illinois University