Enhancing the Breadth of Efficacy of Therapeutic Vaccines for Breast Cancer
Abstract
During the current funding period, we have started a collaborative effort between the laboratories of Peter Lee at City of Hope National Medical Center, Paul Spellman at Oregon Health Sciences University, and Jill Slansky at University of Colorado Anschutz Medical Campus. Our multi-team project is aimed at enhancing the function of pre-existing antitumor T cells from breast cancer patients. In the first year, we have focused on generating reagents and identifying conditions for experiments that will identify T cell antigens from patients with major subtypes of breast cancer. Towards this goal, we have achieved compliance with the Colorado and the Department of Defense Institutional Review Boards, accrued consented patients for optimization of study parameters, optimized conditions for isolation and confirmed function of T cells from breast cancer patients, finalized the protocol for amplification of T cell receptor RNA, sequenced the TCR gene fragments from matched tumor infiltrating and blood T cells, and started to generate an HLA-peptide library. We are positioned to start validating antigens and developing antigenic peptides recognized by T cells from breast cancer patients.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2012
- Accession Number
- ADA569513
Entities
People
- Daniel Munson
- Jill E Slansky
- John Kappler
- Taizo Nakano
- Tullia C. Bruno
Organizations
- University of Colorado Boulder