Systemic and Local Vaccination against Breast Cancer with Minimum Autoimmune Sequelae

Abstract

Our goal is to eliminate the tumor by vaccination and local ablation to render long-term immune protection without excessive autoimmune sequelae. Complimenting this regimen is systemic modulation of natural/induced Treg (iTreg) and intratumoral expression of immune augumenting cytokines. The two aims are to (1) Test the hypothesis that cryosurgery of cytokine enriched tumors amplifies Her-2 vaccine response, and (2) Test the hypothesis that disabling iTreg conversion enhances Her-2 immunity, not autoimmunity. In aim 1, we will measure immune response to tumor associated Her-2 by cryotherapy with and without DNA vaccination and the effect of intratumoral expression of cytokine. In aim 2, we will evaluate the degree of iTreg conversion in tumor bearing mice, measure Her-2 vaccine response in Her-2+TIEG1-/- mice which do not generate iTreg, and measure the induction of experimental autoimmune thyroiditis in iTreg deficient mice.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2012
Accession Number
ADA570213

Entities

People

  • Wei-Zen Wei

Organizations

  • Wayne State University

Tags

DTIC Thesaurus Topics

  • Ablation
  • Antibodies
  • Autoimmunity
  • Biomedical Research
  • Breast Cancer
  • Cells
  • Conversion
  • Cytokines
  • Immunity
  • Lymphocytes
  • Modulation
  • Neoplasms
  • Surgical Ablation Techniques
  • Therapy
  • Vaccination
  • Vaccines

Fields of Study

  • Medicine

Readers

  • Immunology
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech