Role of Mitochondrial Inheritance on Prostate Cancer Outcome in African American Men
Abstract
We are examining the hypothesis that mitochondrial inheritance plays a significant role in aggressiveness of prostate cancer in African Americans. In the first year of the project we have identified 2,000 noncancerous tissues samples from African American men with prostate cancer and we have extracted DNA from ~ 1,500 of them to date. We have validated a robust new DNA sequencing technique developed by our collaborator using single amplicon long-range PCR that permits deep coverage (10,000-20,000X on average) of the mitochondrial genome. We have more than 300 samples in the sequencing pipeline with several hundred more to be submitted in the next few weeks. We have begun mapping of DNA variants in our sequenced genomes to mitochondrial genes. Illumina African Admixture mapping of the initial 72 subjects has been accomplished. Initial steps toward creating the Rho0 cell lines necessary for cybrid generation have begun with generation of puromycin resistant cell lines and testing of two different technologies for eliminating mitochondria from the nuclear donor cell lines.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2012
- Accession Number
- ADA570546
Entities
People
- Martha Mims
Organizations
- Baylor College of Medicine