Rapid Parallel Screening for Strain Optimization
Abstract
Progress has been made on identifying biosensors that will be used to report on the fermentation yields of industrially relevant biological compounds. Screening of the desired chemicals has been largely completed. Microbes that can utilize these compounds as their sole carbon source have been isolated and archived. Phylotyping of the isolated microbes is nearly finished and genomic DNA has been prepared for sequencing. In this reporting period, we have completed the effort to screen the final 20 compounds from the initial set of chemicals. This set of 20 chemicals is more cumbersome than many of those already processed due to their physical properties (poor water solubility, etc). Most of these compounds were successfully used to isolate microbes that can utilize the chemicals as their sole carbon source. In total, almost 70 compounds have been paired with microbes as suggested by colony morphology. We have completed clonal purification, generated freezer stocks, phylotyped, and isolated genomic DNA for these. Growth screens for a final batch of 33 chemicals are underway and we expect to have these ready for sequencing in a couple of weeks. Except for the last batch of chemicals, phylotyping has been completed and we are currently analyzing the data. Genomic DNA from the isolated organisms has been prepared and will be submitted for sequencing on the Illumina HiSeq platform.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 16, 2012
- Accession Number
- ADA570551
Entities
People
- Chuck Merryman
- Tony Yee
Organizations
- J. Craig Venter Institute