Hydrogen-bonded LbL Shells for Living Cell Surface Engineering
Abstract
We report on the design of cytocompatible synthetic shells from highly permeable, hydrogen-bonded multilayers for cell surface engineering with preservation of long-term cell functioning. In contrast to traditional polyelectrolyte layer-by-layer (LbL) systems, shells suggested here are based on hydrogen bonding allowing gentle cell encapsulation using non-toxic, non-ionic and biocompatible components such as poly(N-vinylpyrrolidone) (PVPON) and tannic acid (TA) which were earlier exploited on abiotic surfaces but never assembled on cell surfaces. Here, we demonstrate that these LbL shells with higher diffusion facilitate outstanding cell survivability reaching 79% in contrast to only 20% viability level achieved with ionically paired coatings. We suggest that the drastic increase in cell viability and preservation of cell functioning after coating with synthetic shell stems from the minimal exposure of the cells to toxic polycations and high shell permeability.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 21, 2011
- Accession Number
- ADA571210
Entities
People
- Irina Drachuk
- Morley Stone
- Nancy Kelley-Loughnane
- Olga Shchepelina
- Svetlana V Harbaugh
- Veronika Kozlovskaya
- Vladimir V. Tsukruk
Organizations
- Air Force Research Laboratory