Human Neural Cell-Based Biosensor

Abstract

Our goal is to provide a more biologically relevant model of human physiology for the purpose of developing in vitro, cell based biosensors for environmental toxins. Specifically we plan to develop human neural based biosensors by using ArunA's neural cell lines derived from both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). This report describes the progress made in these major areas: (1) directed differentiation of hESC- and hiPSC-derived neural progenitor cells into dopaminergic neurons; (2) directed differentiation of hESC- and hiPSC-derived neural progenitor cells into astrocytes; (3) label-free, adhesive signature-based microfluidic cell separation; and (4) neural crest differentiation.

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Document Details

Document Type
Technical Report
Publication Date
Dec 07, 2012
Accession Number
ADA571881

Entities

People

  • Steven L. Stice

Tags

DTIC Thesaurus Topics

  • Astrocytes
  • Biosensors
  • Cell Line
  • Cells
  • Contracts
  • Culture Techniques
  • Department Of Defense
  • Electronic Mail
  • Embryos
  • Military Research
  • Neurons
  • Physiology
  • Stem Cells

Fields of Study

  • Biology
  • Chemistry

Readers

  • Immunology and Pathology
  • Molecular and Cellular Biology
  • Neural Network Machine Learning.

Technology Areas

  • Biotechnology