Proof of Concept to Isolate and Culture Primary Muscle Cells from Northern Elephant Seals to Study the Mechanisms that Maintain Aerobic Metabolism Under the Hypoxic Conditions of Breath-hold Diving
Abstract
LONG-TERM GOALS: To isolate and culture primary muscle cells from the swimming muscles of northern elephant seals. OBJECTIVES: Objective 1. To test the hypothesis that the skeletal muscle cells of diving mammals are preconditioned to function under hypoxic conditions compared to terrestrial models. Specifically, proteins and transcripts associated with increased oxygen storage, lipid catabolism and hypoxic pathways will be upregulated in seal primary cells as compared to terrestrial models. Objective 2. To test the hypothesis, that varying levels of stimulation and/or hypoxic conditions will differentially regulate the expression of myoglobin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 30, 2012
- Accession Number
- ADA573541
Entities
People
- Shane B. Kanatous
Organizations
- Colorado State University