Fluorescence Assay Based on Aptamer-Quantum Dot Binding to Bacillus thuringiensis Spores

Abstract

A novel assay was developed for the detection of Bacillus thuringiensis (BT) spores. The assay is based on the fluorescence observed after binding an aptamer-quantum dot conjugate to BT spores. The in vitro selection and amplification technique called SELEX (Systematic Evolution of Ligands by EXponential enrichment) was used in order to identify the DNA aptamer sequence specific for BT. The 60 base aptamer was then coupled to fluorescent zinc sulfidecapped, cadmium selenide quantum dots (QD). The assay is semi-quantitative, specific and can detect BT at concentrations of about 1,000 colony forming units/ml.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2007
Accession Number
ADA575313

Entities

People

  • Amity Allman
  • Carrie J. Andrews
  • John G Bruno
  • Johnathan L. Kiel
  • Maria P. Carrillo
  • Milada Ikanovic
  • Poornima Rao
  • Sulatha Dwarakanath
  • Suneetha Bhahdigadi
  • Walter E. Rudzinski

Organizations

  • Texas State University

Tags

DTIC Thesaurus Topics

  • Air Force
  • Air Force Research Laboratories
  • Chemical Synthesis
  • Chemistry
  • Compound Semiconductors
  • Crystals
  • Detection
  • Emission Spectra
  • Fluorescence
  • Military Research
  • Nanocrystals
  • Quantum Dots
  • Semiconductor Devices
  • Semiconductors
  • Sequences
  • Spores

Fields of Study

  • Biology
  • Chemistry

Readers

  • Microbial Pathology
  • Molecular Genetics

Technology Areas

  • Quantum Computing