The Importance of Neurogenic Inflammation in Blast-Induced Neurotrauma
Abstract
This project addresses the hypothesis that, in response to blast, blood-borne immune cells along with their secreted cytokines and chemokines from the periphery migrate via blood and infiltrate the CNS where they contribute to neuronal damage caused by activated microglia both in acute and chronic injury phases of blast-induced neurotrauma (BINT). To address the scientific feasibility of the hypothesis, we use state-of-the-art imaging and molecular techniques in mice with mild/moderate blast injury generated in a compressed helium-driven shock tube, throughout a one-month observation period. Progress & Results: At assigned time points after injury separate groups of animals with mild/moderate BINT are imaged by magnetic resonance imaging (MRI) to visualize potential macrophage infiltration; blood-brain barrier (BBB) disturbance; reactive gliosis; or astrocyte activation. The imaging findings were validated by immunocytochemistry. The obtained data suggest that a single exposure to mild/moderate blast induces both acute and chronic glial activation, levels of cytokines/chemokines, and motor impairment.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2013
- Accession Number
- ADA575599
Entities
People
- Michele L. Schaefer
Organizations
- Johns Hopkins University