Analysis of Small Intestinal Microbiome in Children with Autism

Abstract

The goal of this project is to analyze the entire microbial population of the mucosa in the upper gastrointestinal tract of children with autism to determine if there is an overgrowth of specific populations of bacteria and analyze the relationship between the intestinal flora and behavior. Our current sub-contractor from the institute of Genome Sciences, University of Maryland School of Medicine was not able to amplify and sequence DNA from the duodenal biopsies of autistic children and controls. A new sub-contractor the Research and Testing Laboratory was found. A new set of duodenal biopsies from children with and without autism will be sent to the Research and Testing Laboratory for DNA extraction, PCR for 16S rRNA, sequencing. No additional funding will be requested to complete this project.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2013
Accession Number
ADA575715

Entities

People

  • Rafail Kushak

Organizations

  • Massachusetts General Hospital

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Anatomy
  • Bacteria
  • Biological Sciences
  • Biomedical Research
  • Biorepositories
  • Contractors
  • Department Of Defense
  • Digestive System
  • Electronic Mail
  • Extraction
  • Gastrointestinal Tract
  • Maryland
  • Microbiomes
  • Schools
  • Sequences
  • Universities
  • Upper Gastrointestinal Tract

Fields of Study

  • Biology

Readers

  • Child and Adolescent Substance Abuse Science in Autism Spectrum Disorders.
  • Immunology
  • Systems Analysis and Design

Technology Areas

  • Biotechnology