The Fate of Intrapleurally Injected Bone Marrow-Derived Stem Cells in Mice with Pleural Mesothelioma

Abstract

This research contract focused on the utility of genetically-engineered bone marrow stem cells (BMSCs) as potential therapeutics against malignant mesothelioma (MM). Our over-arching goal was to determine whether fluorescently-tagged murine BMSCs introduced into a mouse model for MM would home to and infiltrate diseased and normal tissues of interest. Protocols for the isolation, amplification, characterization, and intrapleural injection of fluorescently-tagged BMSCs were developed and optimized where possible. A number of significant technical challenges were encountered, including limiting numbers of viable BMSCs, contamination of BMSC cultures, and histocompatibility issues following intrapleural injection. A fully syngeneic approach was adopted to ameliorate some of these concerns, and injected animals were characterized by indirect immunofluorescence for the presence of BMSCs as well as histopathological changes. Unfortunately, BMSC injection had little or no significant gross effect on mesothelioma progression or pathology.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2012
Accession Number
ADA578223

Entities

People

  • Jonathan M. Horowitz

Organizations

  • North Carolina State University

Tags

DTIC Thesaurus Topics

  • Anatomy
  • Antineoplastic Agents
  • Biological Sciences
  • Biomedical Research
  • Bone Marrow
  • Bones
  • Cancer
  • Cells
  • Department Of Defense
  • Genetically Modified Organisms
  • Medical Personnel
  • Mesothelioma
  • Military Personnel
  • Neoplasms
  • North Carolina
  • Stem Cells
  • Therapy

Fields of Study

  • Biology

Readers

  • Immunology and Pathology
  • Oncology
  • Trauma Surgery or Emergency Medicine.

Technology Areas

  • Biotechnology