Construction of a Bacterial Cell that Contains Only the Set of Essential Genes Necessary to Impart Life
Abstract
Work has continued on the Top Down approach to genome minimization. Because the smallest genome to date grows too slowly to be used in future experiments, we have conducted additional transposon studies and used the data to construct a new, fast growing parental strain. The effort to modularize the genome has been initiated. We have made a modular map of the genome by grouping genes according to function. In the future, we will modularize the genome as much as possible, although we expect that there will be some general genome layout requirements that will make full modularization impossible. Preliminary work aimed at genome complementation has been conducted. A plasmid system for quickly adding back deleted genes will allow us to quickly examine which gene(s) within a deleted cluster might be causing growth defects or cell death.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 16, 2013
- Accession Number
- ADA578874
Entities
People
- John I Glass
- Tony Yee
Organizations
- J. Craig Venter Institute