Identification of Androgen Receptor-Regulated Genes in Castration-Recurrent Prostate Cancer
Abstract
Recent data indicate that castration-recurrent prostate cancer (CR-CaP) progression is driven by the activation of wild-type androgen receptor (AR) through at least two mechanisms: tyrosine phosphorylation by Src family (SFK) and Ack1 tyrosine kinases, and the induction of AR coregulators that regulate the transcriptional activity of AR. It is likely that identifying novel AR-regulated genes in CR-CaP, especially those involving promoters with novel target sequences, will help elucidate the molecular mechanisms that drive CR-CaP initiation and progression, and will help identify potential new therapeutic targets for CR-CaP. We propose to use ChIP-seq, exome-seq and bioinformatics analyses to comprehensively identify AR-regulated genes that drive the growth of human CR-CaP tumors in mice. The long-term aim of this work is to develop a CR-CaP progression gene signature, to identify CR-CaP-associated AR binding site motifs, and to identify potential new therapeutic targets in CR-CaP.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2013
- Accession Number
- ADA579454
Entities
People
- Irwin H Gelman
Organizations
- Health Research, Incorporated