Identification and Characterization of Post-Translational Modifications on EAF1 and EAF2 in Prostate Cancer
Abstract
The project was to determine how the tumor suppressor ELL-associated factor 2 (EAF2) interacts with Fork-head box protein A1 (FOXA1) and the effects of that interaction on prostate cell transcription, growth, and survival. Specific aim 1 is to determine how FOXA1 interacts with EAF2 and the specific aim 2 is to determine the effect of EAF2 on FOXA1-mediated transcription and on FOXA1-mediated cell growth and survival. Work on specific aim 1 revealed that over-expressed FOXA1 binds to EAF2 and FOXA1 does not affect EAF2 stability in the presence of cycloheximide. Work on specific aim 2 revealed that EAF2 alleviates FOXA1-mediated repression of androgen receptor transactivation. Future work will determine which amino acid stretches are required for binding, test if EAF2 can alleviate FOXA1-mediated repression of transcription in the absence of EAF2 binding to FOXA1, and investigate if EAF2 reduces FOXA1-mediated cell growth and survival.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2013
- Accession Number
- ADA580238
Entities
People
- Anne Lipton
Organizations
- University of Pittsburgh