Advanced Imaging Approaches to Characterize Stromal and Metabolic Changes in In Vivo Mammary Tumor Models
Abstract
The purpose of this study is to investigate the connection between cancer cell progression and key factors in the cellular microenvironment such as metabolism and changes in the ECM, specifically by examining the effects of collagen density on cellular metabolism in breast cancer cells. Normal (MCF10A) and invasive (MCF10-Ca1d) breast epithelia cells were cultured in 2D and, to be more relevant, in 3D by suspending the cells in high and low density collagen gels. To stress cellular metabolism, the cells were treated with either DFOM, to mimic a hypoxic response, or 2DG to induce a hypoglycemic environment. Fluorescence lifetime data were then collected for the NADH within the cells. In 2D, the MCF10A cells show the trend of having a shorter free fractional component than MCF10-Ca1d cells. In 3D, increasing collagen density caused an increase in the free fraction of NADH for nearly all cell type and treatment conditions. In most cases DFOM or 2DG caused a decrease in the free fraction of NADH. This in vitro data provides valuable FLIM characterization and toward future intravital studies. Preliminary fluorescence lifetime images were also collected intravitally through a mammary imaging window implanted in a female, PyVT positive, Col1a1 heterozygote, mouse.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2013
- Accession Number
- ADA580941
Entities
People
- Pamela R. Young
Organizations
- University of Wisconsin–Madison