Integrated Device for Circulating Tumor Cell Capture, Characterization and Lens-Free Microscopy
Abstract
The detection of circulating tumor cells (CTC) in the peripheral blood of breast cancer patients indicates high metastatic potential and increased morbidity. Development of a cost-effective CTC detection and imaging system can revolutionize the field of cancer prognosis, therapeutic response monitoring and drug development. Our cell-size-based parylene microfilter platform captures CTC from the cancer patients blood cost effectively, where the larger CTC are preferentially retained on the membrane while typical blood cells flow through. Our collaborators (Yang et al, Caltech) have simultaneously developed novel wide field-of-view scanning microscope method. In the study supported by this funding, we aimed to integrate our novel CTC capture microfilter system with the wide field-of-view scanning microscope to permit harvesting and analysis of CTC at high resolution. At the conclusion, we have completed the task of achieving tumor cell capture through parylene-based microfiltration technology for breast cancer with high sensitivity (Aim 1), defining imaging parameters for CTC analysis using the microfilter device (Aim 2), development and evaluation of various novel WFOV methods in response to the established parameters (Aim 3), and finally, we have combined the microfiltration and WFOV microscopy components to create an integrated CTC analysis system (Aim 4).
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2012
- Accession Number
- ADA581028
Entities
People
- Changhuei Yang
- Ram Datar
- Richard Cote
Organizations
- University of Miami