Investigating Genomic Mechanisms of Treatment Resistance in Castration Resistant Prostate Cancer
Abstract
The purpose of this work is to better understand the mechanisms of resistance to androgen biosynthesis inhibitors in men with castration resistant prostate cancer, and to investigate clinical methods of overcoming resistance. Key Accomplishments and Findings to date: CTCs collected in 12 men with abiraterone-na ve mCRPC. These cells are in the process of enumeration, immunocytochemical analysis for expression of prostatespecific cell surface markers, and DNA isolation. CTCs thus far are detectable in >90% of men using the Vitatex VitaCaP assay. CTCs expressing a mesenchymal phenotype are detectable as well as those bearing markers of stemness including CD44. CTCs from this assay are amenable to flow cytometry both for enumeration and for sorting of different CTC subpopulations. Further genomic analysis of CTC subpopulations to be detailed in subsequent updates. Phase II protocol for Dose- Increased Abiraterone Acetate in Men with mCRPC (PI: Friedlander) written, IRB approved, and accruring patients at by UCSF and Oregon Health Sciences University. Phase II protocol of Abiraterone Acetate plus ARN-5 09 in men with mCRPC (PI: Friedlander) completed, approved by UCSF site-review committee, and in further development at UCSF and at Dana Farber Cancer Institute. Integration of both clinical trials with recently awarded Stand Up 2 Cancer West Coast Dream Team castration-resistant prostate cancer biopsy protocol, allowing for even more comprehensive molecular and genomic analysis of mechanisms of abiraterone/ABI resistance.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2013
- Accession Number
- ADA581161
Entities
People
- Terence W. Friedlander
Organizations
- University of California, San Francisco