Systematic Characterization of the Molecular Mechanisms That Regulate and Mediate Alternative Lengthening of Telomeres in Breast Carcinoma

Abstract

Alternative lengthening of telomeres (ALT) is a mechanism utilized by several cancer types to maintain telomeres. The aim of this project is to characterize the mechanisms that mediate and regulate ALT activity in breast cancer, by utilizing proteomic and genomic approaches to describe and perturb ALT+ cells. Gaining this knowledge on ALT activity can promote the development of novel treatment for ALT+ breast tumors, and patients with telomerase inhibitor resistant tumors, while having minimal non-desired effects on the normal cell population that typically has no ALT activity. Here, I report on my progress in calibrating the C-circle assay, which is used to detect ALT activity, to enable rapid and sensitive quantification of the signal. In addition, I report on my findings regarding the involvement of ATRX and DAXX in induction of ALT, which was also included in a recent publication (Lovejoy CA, 2012 PLoS genetics).

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2013
Accession Number
ADA581164

Entities

People

  • Yaara Zwang

Organizations

  • Dana–Farber Cancer Institute

Tags

DTIC Thesaurus Topics

  • Biology
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Chromosome Aberrations
  • Chromosome Structures
  • Chromosomes
  • Culture Techniques
  • Epithelial Cells
  • Gene Expression
  • Genetics
  • Neoplasms
  • New York
  • Tumor Cell Line
  • United States

Fields of Study

  • Biology

Readers

  • Computational Modeling and Simulation
  • Housing Policy Studies in Military Families with Privatization and Telomerase Allowance Units, Multi-Family Housing, and Telomere Lengths.
  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology