Developing a Mouse Model of Sensory and Cognitive Deficits for Multiple Sclerosis

Abstract

In this project, we have been developing a novel mouse model of multiple sclerosis with a focus on cognitive dysfunction associated with defective myelin. We have developed this model using homologous recombination in embryonic stem cells and have obtained homologous recombinants. However, we have experienced difficulties in deriving mice from the recombinant cells which has delayed our progress toward completion of our goals. We have now repeated the homologous recombinant experiments and are screening for embryonic stem cell clones to be used in homologous recombinant injections. In addition to generation of our novel model, we have been developing neurophysiological techniques to assess cognitive deficits in our novel mouse model. These experiments involve using the auditory pathway to test neural processing in the superior olivary complex, and are proceeding well.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2012
Accession Number
ADA581496

Entities

People

  • Alexander Gow
  • Betty Diamond

Organizations

  • Wayne State University

Tags

DTIC Thesaurus Topics

  • Abnormalities
  • Anesthesia
  • Auditory Signals
  • Cells
  • Diseases And Disorders
  • Dysfunction
  • Ear
  • Electrical Resistance
  • Electroencephalography
  • Intercellular Junctions
  • Medical Personnel
  • Multiple Sclerosis
  • Neural Pathways
  • Physical Disabilities
  • Rodents
  • Sclerosis
  • Stem Cells

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Neuroscience

Technology Areas

  • Biotechnology