Using 3-D Super-Resolution Microscopy to Probe Breast Cancer Stem Cells and Their Microenvironment

Abstract

We report the achievements of the second year of our project. These achievements include development of a high-throughput platform consisting of an array of microposts of different size to investigate the effect of stiffness range on breast-cancer phenotype (given that tumors are heterogeneous in terms of stiffness). We are currently focusing on the MCF-7 a-CD271 phenotype since it has been recently reported to be a candidate breast CaSCs. Through immunostaining, we have identified that phenotype with our MCF-7 cell line. We intend to incorporate microfluidic channels that will deliver specific chemical cues to cells growing on the micropost arrays to best mimic the tumor microenvironment. We will also use our super-resolution microscope developed in Year 1 to image the cells growing to determine spatial pattern changes of surface receptors.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2013
Accession Number
ADA581514

Entities

People

  • Lydia L Sohn

Organizations

  • University of California, Berkeley

Tags

DTIC Thesaurus Topics

  • Aspect Ratio
  • Biomedical Research
  • Breast Cancer
  • Carcinoma
  • Cell Line
  • Cells
  • Department Of Defense
  • Materials
  • Mechanical Engineering
  • Mechanical Properties
  • Medical Personnel
  • Neoplasms
  • Soft Lithography
  • Stem Cells
  • Three Dimensional
  • Tumor Cell Line
  • Two Dimensional

Fields of Study

  • Physics

Readers

  • Nanoscale Plasmonic Nanotechnology
  • Oncology (Cancer Research).
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Biotechnology