Epigenetic Control of Tamoxifen-Resistant Breast Cancer

Abstract

The objective is to understand the role that epigenetics, specifically methylation, plays in antiestrogen resistant breast cancer. The goal of this study is to identify genes differentially methylated between acquired tamoxifen resistant cells (TMX2-28 and TMX2-11) and their parent strain (MCF-7) through the use of the Illumina HumanMethylation450 BeadChip. A panel of genes showing changes in methylation between the tamoxifen resistant cell lines, TMX2-11 and TMX2-28 and the parental line, MCF-7 were selected for further analysis. One gene, ZNF350, which was hypermethylated in both TMX2-11 and TMX2-28 but not in MCF-7, showed significant increase in expression when treated with a demethylase. Additionally, preliminary treatment assays show a decrease in the proliferation rate of TMX2-28 when treated with a demethylase.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2013
Accession Number
ADA581650

Entities

People

  • Kristin P. Williams

Organizations

  • University of Massachusetts Amherst

Tags

DTIC Thesaurus Topics

  • Alkenes
  • Biology
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Department Of Defense
  • Electronic Mail
  • Epigenetics
  • Information Operations
  • Medical Personnel
  • Methylation
  • Neoplasms

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Game Theory.
  • Molecular and genetic basis of cancer.