The Role of Mitochondrial TCA Cycle Enzymes in Determining Prostate Cancer Chemosensitivity
Abstract
Based on the in vitro results in preliminary data and in year #2 of the proposal (1), we conducted an in vivo xenograft mouse experiment using prostate cancer cell lines containing specific levels (endogenous, shRNA, and Flag-MDH2 compensation) of MDH2. We found that xenograft tumors are more sensitive to docetaxel chemotherapy when the endogenous MDH2 is inhibited by shRNA. In contrast, when the loss of MDH2 is compensated by Flag-MDH2 plasmid transfection, the drug sensitivity is lost. This suggests that MDH2 is important to in vivo chemotherapy sensitivity and resistance.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2013
- Accession Number
- ADA581651
Entities
People
- David Qian
Organizations
- Oregon Health & Science University