Search and Neutralize Factors (CSPGs) that Induce Decline in Transmission to Neurons from Spared Fibers after Chronic Spinal Cord Injury
Abstract
During Year 2 we have used anti-NG2-antibodyes made in Dr. Levine's lab and accomplished following studies. 1) Experiments described in Specific Aim (SA)2: we demonstrate that a single intraspinal injection of anti-NG2- antibody (Ab) removes conduction block and restores transmission deficits induced by acute NG2. 2) Experiments described in SA3 and SA4 related to chronic effects of NG2-Ab delivered via osmotic minipump: we demonstrate that prolonged infusion of anti-NG2-Ab strengthens transmission, promotes anatomical plasticity (SA3) and improves locomotor function and coordination in automated Catwalk gait (SA4) after chronic hemisection SCI. 3) We have succeeded to create the recombinant single chain (scFv) antibody and confirmed expression of anti-NG2- Ab; at present, AAV10 encoding the anti-NG2 scFv is being constructed in PENN vector core. During year 3 we will examine functional effects of this AAV-NG2-Ab construct, and accomplish SA3 and 4. If successful, this AAV-NG2- Ab construct will be the novel effective and clinically relevant treatment to facilitate recovery after SCI. Four papers have been published during 2011-2012 period; two other papers have been submitted for publication.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2012
- Accession Number
- ADA581763
Entities
People
- Victor Arvanian