Cryogenic Collection of Complete Subsurface Samples for Molecular Biological Analysis

Abstract

The objective of this project has been to demonstrate the efficacy of cryogenic core technology as an integrated approach to sample collection and handling for use as a tool for monitoring subsurface remediation. This has included the development of a in situ cryogenic coring system, the evaluation of molecular biological tools (MBTs) analysis in conjunction with that system and the demonstration that the cryogenic sampling protocols are robust and ready for application by the remediation user community. In addition, cryogenic sample handling has allowed development of a new approach to directly determine local degradation activity using the ratio of labile mRNA to stable DNA for target genes.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2012
Accession Number
ADA581958

Entities

People

  • Christina N. Brow
  • Holly S. Simon
  • Reid O. Johnson
  • Richard L. Johnson

Organizations

  • Oregon Health & Science University

Tags

DTIC Thesaurus Topics

  • Alkenes
  • Bacteria
  • Bacteriology
  • Biodegradation
  • Chemical Analysis
  • Chemical Kinetics
  • Chemical Reactions
  • Chemical Synthesis
  • Chemistry
  • Climate Change
  • Detection
  • Ecology
  • Environment
  • Environmental Protection
  • Groundwater
  • Microbiology
  • Microorganisms

Readers

  • Groundwater Contamination Remediation.
  • Molecular Genetics
  • Software Engineering.