Quantitative Analysis, Design, and Fabrication of Biosensing and Bioprocessing Devices in Living Cells

Abstract

This project aims at designing sensing systems in bacteria E. coli by employing and re-engineering components from natural sensing systems. As shown in Figure 1, any such sensing system must have a detector, a transmission system, and a computation element, which produces a visible output. The transmission system usually involves covalent modification cycles such as phosphorylation (the MAPK cascades), while the computation element usually involves gene expression. The properties that we look for in a sensing system are (a) high sensitivity to the presence of molecules to be sensed and (b) fast response time so that the visible output is displayed with minimal delay with respect to when the environmental molecule appeared.

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Document Details

Document Type
Technical Report
Publication Date
Aug 29, 2012
Accession Number
ADA582056

Entities

People

  • Domitilla Del Vecchio

Organizations

  • Massachusetts Institute of Technology

Tags

Communities of Interest

  • Biomedical
  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Biology
  • Biosensors
  • Biotechnology
  • Computational Biology
  • Computations
  • Detectors
  • Dynamic Response
  • Engineering
  • Fabrication
  • Gene Expression
  • Information Processing
  • Molecules
  • Phosphorylation
  • Signal Processing
  • Steady State
  • Synthetic Biology
  • Systems Biology

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