Characterisation and Next-generation Sequencing Analysis of Unknown Arboviruses

Abstract

In the last few years there has been an increased incidence of neurological and encephalitic syndromes in Australia that have not been linked to a known pathogen. Viruses from the families Rhabdoviridae and Bunyaviridae are postulated to play a role. This report describes the characterisation of two unknown bunyaviruses, designated Maprik (MPKV) and Buffalo Creek (BCV), and two unknown rhabdoviruses, designated Harrison Dam (HARDV) and Holmes Jungle (HOJV), using techniques such as PCR-select subtraction and next-generation sequencing. Preliminary analysis of the four sequenced viruses has shown that they are all novel, and phylogenetic studies will now be performed to further classify these organisms. In addition, the report details the success in using a novel method to prepare MPKV and BCV RNA for next-generation sequencing. The use of this method allows for an increased level of efficiency when sequencing unknown viruses, and has now been incorporated into our standard viral preparation protocols.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2012
Accession Number
ADA582386

Entities

People

  • Ania Gubala
  • Jane Mcallister
  • Penny Gauci

Organizations

  • Defence Science and Technology Group

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Arbovirus Infections
  • Arboviruses
  • Biological Sciences
  • Biotechnology
  • Bunyaviridae
  • Chemical Synthesis
  • Chemistry
  • Diseases And Disorders
  • Encephalitis
  • Equine Encephalitis
  • Health Services
  • Identification
  • Medical Personnel
  • Rift Valley Fever
  • Rna Viruses
  • Standards
  • Viruses

Fields of Study

  • Biology

Readers

  • Molecular and genetic basis of cancer.
  • Systems Analysis and Design
  • Virology (or Medical Virology).