Assessment of Geographical Variation in the Respiratory Toxicity of Desert Dust Particles

Abstract

The health consequences of sand particle inhalation are incompletely understood. This project evaluated the respiratory toxicity of sand particles collected at military bases near Fort Irwin USA, in Iraq (Camp Victory, Taji and Talil), and Khost Afghanistan. Our primary focus was on assessing the role of soluble metals in the respiratory toxicity of the sand particles using in vitro and in vivo methods. Replicating rat type II alveolar cell cultures (RLE-6TN) were exposed to sand extracts or vehicle control in serum-free media for 24 h. Cytotoxicity was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and assessment of lactate dehydrogenase leakage.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2013
Accession Number
ADA582936

Entities

People

  • David C. Dorman
  • Elisa Fornero
  • J. M. Law
  • Jose A. Centeno
  • Karen Taylor
  • M. S. Henderson
  • Melanie L. Foster
  • Michael G. Stockelman
  • Sabrina A. Trager

Organizations

  • United States Naval Research Laboratory

Tags

DTIC Thesaurus Topics

  • Blood
  • Body Weight
  • Cells
  • Culture Media
  • Health Services
  • Laboratory Animals
  • Lung Diseases
  • Medical Personnel
  • Middle East
  • Particles
  • Particulate Matter
  • Proteins

Readers

  • Analytical Chemistry
  • Immunology and Pathology
  • Military and Counterinsurgency Studies.