Inactivation of Spores of Bacillus Species by Wet Heat: Studies on Single Spores Using Laser Tweezers Taman Spectroscopy

Abstract

We have investigated the aspects of the release of Ca-dipicolinic acid (DPA), protein denaturation and cellular heterogeneity of single Bacillus spores during wet-heat treatment that are commonly used in spore killing and inactivation. Achievements include: (1) determined kinetic change in spore state and Ca-DPA levels in single spores of Bacillus and Clostridium species during heat activation; (2) measured the rates of Ca-DPA release and protein denaturation of individual spores when incubated in water at elevated temperatures; (3) determined the effects of wet heat-treatment on the germination of individual spores and identified the damages of key proteins; (4) developed useful methodologies to analyze multiple individual spores during wet-heat treatment and germination.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2013
Accession Number
ADA583552

Entities

People

  • Yong-qing Li

Organizations

  • East Carolina University

Tags

DTIC Thesaurus Topics

  • Amorphous Materials
  • Analytical Chemistry
  • Chemistry
  • Electro-Optics
  • Engineering
  • Environment
  • Heat Treatment
  • Light Scattering
  • Nucleic Acids
  • Optics
  • Raman Spectra
  • Raman Spectroscopy
  • Scattering
  • Spectra
  • Spectroscopy
  • Spores
  • Students

Readers

  • Military/Explosive Ordnance Disposal (EOD) Technology
  • Molecular Genetics
  • Powder metallurgy of Titanium alloys.

Technology Areas

  • Directed Energy