Development of Advanced Technologies for Complete Genomic and Proteomic Characterization of Quantized Human Tumor Cells

Abstract

With the establishment of GBM cell lines and quantized cell populations form these GBM patients tumor samples we are able to complete some of our aims of our project. We will continue to collect tumor samples with consent from families of GBM patients in preparation to perform the molecular analysis of these. Our efforts in the development of quantized cells and genomic technology to interpret specific transcriptome signatures from quantized cell populations has been completed and is now applied to several GBM cell lines and their quantized sub-populations. Whole genome sequencing from families from our group is now well established and has been published.. Our efforts in the development of quantitative assays for essentially every human protein have been successfully established. We have established cell culture conditions to enable proteomic analysis of quantized cell sub populations. This program will be will provide new technical capability that has already provide significant results and will be completed over the next 12 months.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2013
Accession Number
ADA583585

Entities

People

  • Robert L Moritz

Organizations

  • Institute for Systems Biology

Tags

DTIC Thesaurus Topics

  • Blood
  • Blood Proteins
  • Cell Line
  • Cells
  • Computational Biology
  • Culture Techniques
  • Demographic Cohorts
  • Gene Expression
  • Health Services
  • Mass Spectrometry
  • Molecules
  • Proteins
  • Proteomics
  • Stem Cells
  • Systems Approach
  • Systems Biology
  • Transcription Factors

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Molecular and Cellular Biology
  • Quantum spin resonance or Electron Paramagnetic Resonance spectroscopy.

Technology Areas

  • Biotechnology