Novel Strategy for Lineage Tracing of Cancer Stem Cells by Lentiviral Barcoding

Abstract

The cancer stem cell theory posits a subpopulation of tumorigenic cells with self-renewal potential and the capability to wholly reconstitute original tumors by lineage restriction. We proposed a novel lentiviral tagging system to genetically trace immunosorted cell fate, and further investigate relative TIC enrichment through immunosorting protocols. We have developed a genetic-based strategy to dramatically improve sensitivity and experimental time in prospective identification of novel biomarkers. In months 25-36, we build on our previous results and implemented next-generation sequencing methodology to further trave the tumor sublineages in the mouse mammary tumor model.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2012
Accession Number
ADA583773

Entities

People

  • Edmund Chang

Organizations

  • Baylor College of Medicine

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Biological Markers
  • Biomedical Research
  • Cells
  • Department Of Defense
  • Electronic Mail
  • Governments
  • Heterogeneity
  • Information Operations
  • Instructions
  • National Governments
  • Personal Information Managers
  • Radiation
  • Sensitivity
  • Sequences
  • Stem Cells
  • Transplants

Fields of Study

  • Biology

Readers

  • Oncology (Cancer Research).
  • Oncology and Biomarker-Based Cancer Detection.
  • Systems Analysis and Design

Technology Areas

  • Biotechnology