Understanding and Targeting Cell Growth Networks in Breast Cancer

Abstract

In this third annual review, we have demonstrated that NPM and eEF1a1 5' TOP mRNAs are translationally repressed by rapamycin. The eEF1a1 5' UTR, but not the NPM 5' UTR, is sufficient to confer rapamycin sensitivity to luciferase. The NPM 5' TOP motif is neither necessary nor sufficient for growth-dependent translational control of the NPM mRNA. The eEF1a1 5' TOP motif functionally dominates the NPM TOP. ARF impairs association of DDX5 with the nuclear pre-ribosome fractions. Overexpression of DDX5 promotes ribosome output. ARF overexpression and DDX5 knockdown each reduce the cytosolic polysome profile in a p53-independent manner. DDX5 is a crucial non-oncogene in human breast cancer. Taken together, these insightful findings bring us significantly closer to our goal of understanding how signaling pathways impact major tumors suppressors in the control of cell growth.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2011
Accession Number
ADA585055

Entities

People

  • Jason D Weber

Organizations

  • Washington University in St. Louis

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amplification
  • Biogenesis
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Carrier Proteins
  • Cell Line
  • Cells
  • Coding
  • Epithelial Cells
  • Genetics
  • Growth Factors
  • Microarray Analysis
  • Neoplasms
  • Organelles
  • Production
  • Proteins

Fields of Study

  • Biology

Readers

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  • Molecular and genetic basis of cancer.
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