ICBP90 Regulation of DNA Methylation, Histone Ubiquitination, and Tumor Suppressor Gene Expression in Breast Cancer Cells

Abstract

In mammals and other higher eukaryotes, SRA-RING proteins are essential for global maintenance of DNA methylation. This research project focuses on the discovery of new ubiquitination targets for the Arabidopsis SRA-RING protein VIM1 and the human SRA-RING protein UHRF1. Additionally, it will closely examine the methylcytosine-binding specificity of UHRF1, with a specific focus on non-CpG contexts. The proposed work is ongoing, and so far the major accomplishments include creation of relevant plant lines, development of in vitro assays, and profiling of mRNA expression in null mutants.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2013
Accession Number
ADA586207

Entities

People

  • Elena N. Cravens
  • Eric J. Richards
  • Erika J. Hughes
  • Molly S. Shook
  • Scott A. Coonrod

Organizations

  • Cornell University

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Chromosome Structures
  • Department Of Defense
  • Drug Receptors
  • Epigenetics
  • Gene Expression
  • Genetics
  • Genomics
  • Maintenance
  • Methylation
  • Neoplasms
  • Proteins
  • Proteomics

Readers

  • Molecular Biology and Genetics
  • Molecular Genetics
  • Space Exploration and Orbital Mechanics.