T Cell Lipid Rafts and Complement Ligands for Diagnosis and Monitoring
Abstract
Previously we documented that T cells from normal individuals express complement on the surface membrane after stimulation. We discovered there is a significant difference in the distribution of complement proteins on T cells from SLE patients compared to other autoimmune diseases and normal individuals and that complement deposition (C4d and C3d) is associated with SLE disease activity. We also discovered that complement deposition affects T cell function and decrease (or goes along with) Ca flux, IL- 2 production and cytotoxic activity. During this past year the following has been accomplished. 1. C4d bound to critical surface membrane proteins of SLE T cells Lipid rafts and CD3. 2.C4d is associated with aberrant signal transduction and additional downstream effects, which in turn may contribute to T cell dysfunction and overall abnormalities of the immune system in SLE patients. 3.There is increased cytokine production by C4d + SLE T cells. 4.There is increased cytokine production by C3d+ T cells. 5.C3d is partially co-localized within aggregated lipid rafts on a subpopulation of SLE T cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2010
- Accession Number
- ADA587059
Entities
People
- George C. Tsokos
Organizations
- Beth Israel Deaconess Medical Center