The Effect of Smoking on Muscle Adaptation to Exercise
Abstract
Cigarette smoking is prevalent in the military and is associated with increased risk for musculoskeletal injury. The objective of this study is to investigate differences between smokers and non-smokers in their response to a muscle-damaging exercise. Specifically, we measured both functional strength and collected muscle and blood samples for analysis of molecular mechanisms that may impact this response. Ten smokers and ten non-smokers performed a maximal eccentric exercise of the non-dominant knee flexors. Isometric and isokinetic strength were measured pre- and 5min, 1, 4, and 9d post-exercise. Blood was collected pre- and 20h post-exercise, and biopsy samples were obtained from control and exercise legs at 48h post-exercise. In preliminary analyses, we found that smokers had lower baseline strength for isokinetic measures and has greater loss in flexion strength at 4d post-exercise for both isometric and isokinetic tests, significantly for isometric strength. We plan to complete gene expression analysis using PCR arrays targeted at the AKT, VEGF, NOS, and NFkB pathways by mid-January 2011. Protein quantification and localization will be analyzed using Western Blotting and Immunohistochemistry, both of which we aim to complete before March 2011. Blood will be analyzed for cytokines and other endocrine signalers in February and March 2011.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2010
- Accession Number
- ADA587066
Entities
People
- Priscilla M. Clarkson
Organizations
- University of Massachusetts Amherst