Targeted Delivery of Carbon Nanotubes to Cancer Cells
Abstract
Single-walled carbon nanotubes (CNTs) convert absorbed near infrared (NIR) light into heat, which can thermally ablate cells that have bound the CNTs. CNTs coupled to monoclonal antibodies (MAbs) may specifically target and kill cells by non-invasive exposure to near infrared (NIR) light. Here, we describe the preparation of conjugates in which CNTs are either non-covalently or covalently conjugated to the MAbs against human CD22 or CD25. In the first approach, biotinylated polar lipids were used to prepare stable, biocompatible, non-cytotoxic CNT suspensions that were then attached to one of the two different Neutralite Avidin (NA)-derivatized MAbs.. In the second approach the two MAbs were covalently coupled to carboxylated CNTs. The specificity of the binding and killing of the target cells by both types of MAb-CNTs was demonstrated by using CD22+CD25- Daudi cells, CD22-CD25+ phytohemagglutinin (PHA)-activated normal human peripheral blood mononuclear cells (PBMCs). We also demonstrate that the conjugates were stable and active following incubation with mouse serum.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2009
- Accession Number
- ADA587582
Entities
People
- Ellen Vitetta
Organizations
- University of Texas at Dallas