Development and Characterization of Novel Bioluminecent Systems
Abstract
The overall objective of this project was to discover, design and demonstrate the feasibility of bioluminescent materials for use in marking, tagging, and anti-tamper applications.(1) The technology for near-Infrared (nIR) dye labeling and (BRET) activation of a firefly luciferase were transferred to Rajesh Naik's lab at AFRL.(2) The construction and evaluation of novel protease substrates (luciferase fusion proteins emitting nIR light) for caspase and the two blood clotting factors was completed.(3) Novel nIR emitting quantum dots/rods were made using luciferases in collaboration with Mathew Maye, Syracuse University. (4) A firefly luciferin benzothiophene-containing analog with glow kinetics and 523 nm emission was discovered. (5) Analyses of luminescent materials from C. variopedatus provided by the Deheyn lab revealed the presence of riboflavin.(6) A firefly luciferase variant was intramolecularly cross-linked trapping it in a C-domain rotated previously undocumented conformation. (7) With A. Gulick (Hauptman Woodward Institute) luciferase crystal structures were determined providing insight into the mechanism of the oxidative light reaction.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2013
- Accession Number
- ADA588105
Entities
People
- Bruce R Branchini
Organizations
- Connecticut College