iPSC-Derived MSCs that Are Genetically Engineered for Systemic Bone Augmentation

Abstract

Mesenchymal stem cells (MSCs) differentiated from Induced pluripotent stem cells (iPSCs) have a potential application in clinic to treat osteoporosis and other skeletal diseases. Further engineering MSCs with homing factors like CXCR4 and osteogenic factors like shNoggin or FGF2 may increase the therapeutic effects. Toward these goals, we have generated iPSCs using lentiviral vectors from blood cells and integration-free iPSCs using episomal vectors. The generated iPSCs are pluripotent, as evident by expression of pluripotency markers and formation of teratoma. After differentiation of iPSCs into MSCs, the cells express MSC markers and can form bone nodule in in vitro culture. To improve the safety of this therapy, we developed an alternative strategy for generating MSCs: direct conversion of blood cells into MSCs rather than reprogramming blood cells into iPSCs followed by re-differentiation.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2013
Accession Number
ADA588141

Entities

People

  • Xiaobing Zhang

Organizations

  • Loma Linda University

Tags

DTIC Thesaurus Topics

  • Cells
  • Chemistry
  • Confocal Microscopy
  • Cultured Cells
  • Health Services
  • Medical Personnel
  • Regenerative Medicine
  • Stem Cells

Fields of Study

  • Biology
  • Chemistry

Readers

  • Immunology and Pathology
  • Molecular and Cellular Biology

Technology Areas

  • Biotechnology