Co-immobilized Coupled Enzyme Systems in Biotechnology
Abstract
The development of co-immobilized multi-enzymatic systems is increasingly driven by economic and environmental constraints that provide an impetus to develop alternatives to conventional multistep synthetic methods. As in nature, enzyme-based systems work cooperatively to direct the formation of desired products within the defined compartmentalization of a cell. In an attempt to mimic biology, co-immobilization is intended to immobilize a number of sequential or cooperating biocatalysts on the same support to impart stability and enhance reaction kinetics by optimizing catalytic turnover. There are three primary reasons for the utilization of co-immobilized enzymes: to enhance the efficiency of one of the enzymes by the in-situ generation of its substrate, to amplify a process that is conventionally carried out in several steps and/or to eliminate undesired by-products of an enzymatic reaction. As such, co-immobilization provides benefits that span numerous biotechnological applications , from biosensing of molecules to cofactor recycling and to combination of multiple biocatalysts for the synthesis of valuable products.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2010
- Accession Number
- ADA588384
Entities
People
- Heather R. Luckarift
- Lorena Betancor
Organizations
- Air Force Research Laboratory