Toward Development of a Food-Based Genetic Approach to Overcoming Food Allergies
Abstract
Curcuminoids are plant natural products that have shown promising anti-allergic activities. They have been used in Asian traditional medicine and food spice for centuries and have been confirmed to be safe. In this study, we have reconstituted curcuminoid biosynthesis in Escherichia coli by heterologous expression of the biosynthetic enzymes. We have also established an effective expression platform for functional reconstitution of natural product biosynthetic enzymes in lactic acid bacteria (LAB) using green fluorescent protein as a reporter. A colony PCR technique has been developed to efficiently screen correct LAB transformants. A series of expression plasmids thatharbor curcuminoid biosynthetic genes and other natural product biosynthetic enzymes has been constructed for expression in LAB.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2012
- Accession Number
- ADA589216
Entities
People
- Jixun Zhan
Organizations
- Utah State University