Nrf2: A Novel Biomarker of Disease Severity and Target for Therapeutic Intervention in Multiple Sclerosis

Abstract

It has been suggested that the molecules capable of inducing endogenous antioxidant enzymes through the activation of the Nrf2/ARE pathway may be highly effective in therapeutic and chemopreventive strategies designed to inhibit pathological processes underlying MS lesion formation. Under this proposal we have employed a successful strategy to isolate and characterize one of such compound Triterpenoid from natural plant resources. By applying diversity oriented synthesis we have created several unique triterpenoid structures which will enable careful dissection of the Nrf2-dependent and independent pathways mediating neuroprotection and inflammation and will define a novel class of potent and effective therapeutic and chemopreventive agents for multiple sclerosis. The following progress has been made within the first year of this project: 1) Successful large scale (in gram quantity) isolation of Bryonolic acid from zucchini plant (Cucurbita pepo L.) roots. 2) Characterized the activity of isolated Bryonolic acid 3) Applied Diversity Oriented Synthesis to Bryonolic acid to rearrange skeletal structure 4) Analyzed the specificity and activity of skeletally diverse triterpenoids in vivo and in vitro.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2013
Accession Number
ADA589739

Entities

People

  • John Letterio

Organizations

  • Case Western Reserve University

Tags

DTIC Thesaurus Topics

  • Anti-Inflammatory Agents
  • Antioxidants
  • Biochemistry
  • Biological Markers
  • Biological Products
  • Cells
  • Chemistry
  • Culture Techniques
  • Diseases And Disorders
  • Inflammation
  • Macrophages
  • Materials
  • Molecules
  • Multiple Sclerosis
  • Nervous System
  • Sclerosis
  • Transcription Factors

Fields of Study

  • Chemistry

Readers

  • Immunology and Pathology
  • Molecular and Cellular Biochemistry

Technology Areas

  • Biotechnology