Modulating Radiation Resistance: Novel Protection Paradigms Based on Defenses against Ionizing Radiation in the Extrempohile Deinococcus radiodurans
Abstract
In 2012-2013, we demonstrated that (1) Mn2+ complexes in living D. radiodurans cells are distinctly different from those in radiosensitive cells. As demonstrated by ENDOR/EPR/ESEEM spectroscopy, Mn2+ in D. radiodurans is bound mainly to nitrogenous ligans, forming complexes with orthophosphate (Pi) which are extremely resistant to gamma-radiation and preserve the D. radiodurans proteome. This work was published in PNAS USA (2013); (2) Many environmental yeast are extremely radiation-resistant, accumulate nitrogenous Mn2+-Pi complexes, and highly resistant to radiation-induced protein oxidation; (3) The radioprotective nature of Mn-peptide complexes is more dependent on the amino acid composition than the order of amino acids. A variety of decapeptide sequences were screened for their ability to protect protein structure and function following exposure to megadoses of ionizing radiation; and (4) The Mn-peptide-Pi vaccine approach developed for methicillin-resistant Staphylococcus aureus (MRSA), published in Cell Host and Microbe (2012), was successfully applied to Venezuelan equine encephalitis virus.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2013
- Accession Number
- ADA589920
Entities
People
- Michael J. Daly
Organizations
- Uniformed Services University of the Health Sciences