White Matter Glia Pathology in Autism
Abstract
The overall goal of this proposed work is to begin elucidating the cellular and molecular basis of white matter abnormalities in autism spectrum disorder (ASD). Using laser capture microdissection (LCM), subpopulations of white matter glia, i.e. astrocytes and oligodendrocytes, can be isolated independently of other brain cells and analyzed for gene expression differences that might contribute to ASD pathology. The combination of LCM and transcriptional analysis offer an innovative approach to determining the cellular basis of brain pathology in ASD. Due to the time intensive nature of LCM, the first year of the grant was set aside primarily for sample collection. During this year, multiple LCM instrument failures have slowed the progress of cell capture. Despite setbacks, we were able to conduct preliminary feasibility studies and precisely determine the course of action to complete the project in the second year of the award. Our university has purchased a new Arcturus XT LCM instrument that is expected to arrive and be functional within the first two weeks of October this year. This annual report summarizes the efforts made to overcome unforeseen obstacles that have modestly impeded the progress of this grant.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2013
- Accession Number
- ADA590212
Entities
People
- Gregory A. Ordway
- Michelle J Chandley
Organizations
- East Tennessee State University