Exosome Mediates Stemness Transfer from Prostate Epithelial Progenitors to Prostate Cancer Cells
Abstract
Exosomes are small membrane vesicles secreted by most cell types, functioning as signal transmitters by conveying their bioactive molecules, such as microRNAs and mRNAs to the neighboring cells. It has been reported that normal prostatic epithelial progenitor cells existing in the prostate basal component possess the regenerating ability. However, the connection between prostatic progenitor cells and prostate stem cell is still elusive. The purpose of this study is to determine whether exosomes derived from prostatic epithelial progenitor cells can transfer stemness molecules from prostatic epithelial progenitor cells to prostate cancer cells to contribute prostate cancer stem cell generation and/or maintenance. For this purpose, WPEstem cells were used as a source of prostatic epithelial progenitor cells. We first demonstrate that exosomes derived WPEstem cells can be taken by prostate cancer cells LNCap. Secondly, microRNA profiling, QPCR, and proteomic analysis reveal that WPE-stem cell derived exosomes can deliver certain stem cell signatures such as SOX2 and FOXC2 to LNCap cells. Finally, we found that exosomes derived from WPE-stem cells increase LNCap cells invasion ability. However, we do not observe significant effect of WPE- stem cell derived exosomes on LNCap cell growth, morphology change, sphere formation and CD44+/ 2 1 hi/CD133+ population.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2013
- Accession Number
- ADA590430
Entities
People
- Fangting Wu